Banca de QUALIFICAÇÃO: VIVIANE FARIA MORAIS JOTTA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : VIVIANE FARIA MORAIS JOTTA
DATE: 24/03/2023
TIME: 13:00
LOCAL: campus gameleira
TITLE:

CARACTERIZAÇÃO E ESTUDO DA TAXONOMIA E DO PERFIL FUNCIONAL DA COMUNIDADE MICROBIANA DE PAINÉIS FOTOVOLTAICOS UTILIZANDO METAGENÔMICA SHOTGUN

KEY WORDS:

Photovoltaic panels; biofilm; metagenomics; shotgun; extremophiles

PAGES: 106
BIG AREA: Ciências Biológicas
AREA: Microbiologia
SUMMARY:

Photovoltaic panels are surfaces that remain exposed to adverse conditions, such as radiation, high temperature variations, oxidative stress, among others, being considered an extreme environment. Nevertheless, these panels are colonized by diverse microbial communities, and may contain bacteria, fungi, viruses and algae. Photovoltaic panels can lose their efficiency due to the presence of these microorganisms on their surface, since they are capable of forming biofilms as defense mechanisms that affect the absorption of radiation. Thus, the study and characterization of the biofilm present in the photovoltaic panel become extremely important. Therefore, the main objective was to characterize and study the microbial community present on the surface of photovoltaic panels located in the city of Belo Horizonte (MG). Initially, nine samples of the photovoltaic panel biofilm were collected, three technical and three biological replicates, and the DNA of the microbial community was extracted. Then, a shotgun sequencing was performed through the Illumina platform and the metagenomes were analyzed with the SqueezeMeta pipeline. The results were analyzed using the R Software, performing analyzes of the taxonomic and functional profile, in addition to statistical analyses. As a result, more than 1000 genera of bacteria, 20 of archaea and 400 of eukaryotes were identified. Several extremophile and EPS-producing genera were identified, such as Hymenobacter, Pedobacter, Mucilaginibacter and Sphingomonas, that are capable of surviving adverse conditions and forming biofilms as a defense mechanism. Considering the functional results, more than 10.000 genes were identified in biofilm samples, many of which are involved in biofilm formation and resistance to radiation, temperature and desiccation. It was possible to identify and characterize the microbial community of the biofilm present on the surface of the photovoltaic panel, as well as to relate the taxonomic and functional analyzes.

COMMITTEE MEMBERS:
Presidente - PRISCILA PEREIRA SILVA CALDEIRA
Externa à Instituição - RAQUEL CARDOSO DE MELO MINARDI