Banca de DEFESA: CARLA ALESSANDRA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CARLA ALESSANDRA SILVA
DATE: 07/12/2023
TIME: 14:00
LOCAL: campus gameleira
TITLE:

STUDY OF PROKARYOTE DIVERSITY IN SAMPLES OF SOIL OF SERRA DA PIEDADE/MG USING METATAXONOMIC APPROACH

KEY WORDS:

DNA analysis. Taxonomic characterization. Molecular Approach. Soil Canga

PAGES: 70
BIG AREA: Ciências Biológicas
AREA: Microbiologia
SUMMARY:

The identification of the biodiversity of microorganism communities has advanced significantly thanks to the use of next generation sequencing techniques, which represent a powerful approach to investigating soil microbial diversity. The objective of this study was to analyze the taxonomic diversity of prokaryotes in soil samples from Campo Rupestre Ferruginoso in a canga ecosystem area of Serra da Piedade. For this, the metabarcoding sequencing technique associated with the 16S rRNA marker was used. The DNA from the samples was extracted and sequenced using the Illumina platform. Furthermore, the samples were subjected to chemical analysis at the Soil Analysis Laboratory - Instituto Mineiro de Agropecuária (LASO-IMA) with the aim of associating the chemical characteristics of the soil with the local microbiota. The bacterial phylum that predominated in the samples were Actinobacteriota (40,7%), Proteobacteria (21,79%), Acidobacteriota (12,5%) and Chloroflexi (11,73%). Furthermore, the most abundant genus was Conexibacter (17,28%), Acidothermus (15,39%) and Bryobacter (12,04%). The genus Conexibacter has a remarkable ability to degrade complex organic compounds, including lignocellulose. This makes it a valuable source of enzymes involved in the degradation of plant biomass, an essential resource for the production of biofuels and green chemicals. Furthermore, its ability to adapt to challenging soils expands its biotechnological potential in bioremediation and pollutant degradation processes. The genus Acidothermus and Bryobacter are resistant to extreme conditions, such as high temperature and low nutrient availability, in addition to the deposition of complex organic matter. Chemical analysis of the soil revealed that pH values in water indicate high acidity, which may be related to high levels of Al3+. In acidic soils, the release of aluminum ions from minerals present in the soil is common, making these soils profoundly dystrophic. In summary, this research has highlighted the importance of metataxonomic approaches for understanding soil microbial diversity, as it represents a significant advance in the ability to identify and analyze diversity in soil microorganism communities and has provided valuable insight into the composition and structure of microorganism communities in this unique ecosystem in Serra da Piedade/MG, demonstrating the biotechnological potential of these microorganisms especially in the context of biomass degradation, enzyme production and bioremediation. These results lay a solid foundation for future investigations into microbial ecology, nutrient cycling, and ecosystem functioning in this challenging environment.

COMMITTEE MEMBERS:
Presidente - FERNANDA BADOTTI
Interna - ANDREA RODRIGUES MARQUES
Interno - CLEVERSON FERNANDO GARCIA
Externa ao Programa - FATIMA DE CASSIA OLIVEIRA GOMES